p ikba  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Human iPSC-derived CNS and retinal microvasculature-on-a-chip models recapitulate hallmarks of diabetic microvascular pathology

doi: 10.64898/2026.01.15.699708

Figure Lengend Snippet: Gene expression changes in CNS-mv-on-a-chip induced by high glucose level. (A) The principal component analysis (PCA) plot shows clustering of samples cultured under physiological (5.5 mM glucose) and hyperglycemic (75 mM glucose) conditions. (B) Volcano plot illustrating differentially expressed genes between physiological and hyperglycemic CNS-mv-on-a-chip conditions. Orange and blue dots represent significantly upregulated and downregulated genes, respectively (FDR < 0.05, |FC| >2). (C) Heatmap showing upregulated genes associated with inflammation. Values are shown as fold changes relative to control group mean. (D) Over-representation analysis (ORA) plot of genes upregulated under hyperglycemia conditions. (E) Quantitative PCR analysis of IL1B, JUN and NFKBIA expression in ECs and PCs isolated from CNS-mv-on-a-chip cultured under physiological 5.5 mM glucose concentration (5.5G), hyperglycemic conditions 25 mM (25G) and 75 mM glucose (75G), or corresponding osmotic controls with mannitol (19.5M and 69.5M). Gene expression was normalized to GAPDH and HMBS as housekeeping genes and calculated using the ΔΔCt method. Data are presented as mean ± SD, n = 3-4.

Article Snippet: First, a pre-amplification step with TaqMan primers (Thermo Fisher Scientific) was carried out for GAPDH (Hs99999905_m1), HMBS (Hs00609297_m1), IL1B (Hs01555410_m1), JUN (Hs01103582_s1), and NFKBIA (Hs00355671_g1).

Techniques: Gene Expression, Cell Culture, Control, Real-time Polymerase Chain Reaction, Expressing, Isolation, Concentration Assay